10 proteins mix  (Bio-Rad)

Journal: Molecules

Article Title: Stable Isotope Labeling-Based Nontargeted Strategy for Characterization of the In Vitro Metabolic Profile of a Novel Doping BPC-157 in Doping Control by UHPLC-HRMS

doi: 10.3390/molecules28217345

Figure Lengend Snippet: Confirmation of M3 based on synthetic RMs. ( a ) Extracted ion chromatogram (EIC) of m / z 575.77238 (M3) in human liver microsomes incubated sample; ( b ) EIC of the RM BPC-157 (2–13) free acid; ( c ) EIC of the RM BPC-157 (1–12) free acid; ( d ) MS/MS spectrum of m / z 575.77238 (M3); ( e ) MS/MS spectrum of the RM BPC-157 (1–12) free acid; ( f ) MS/MS spectrum of the RM BPC-157 (2–13) free acid.

Article Snippet: Mixed-gender human liver microsomes (10-Donor Pool, total protein concentration of 20 mg/mL) and mixed-gender human skin S9 (3-Donor Pool, total protein concentration of 1.89 mg/mL) were purchased from BioreclamationIVT (Westbury, NY, USA).

Techniques: Incubation, Tandem Mass Spectroscopy

Journal: Molecules

Article Title: Stable Isotope Labeling-Based Nontargeted Strategy for Characterization of the In Vitro Metabolic Profile of a Novel Doping BPC-157 in Doping Control by UHPLC-HRMS

doi: 10.3390/molecules28217345

Figure Lengend Snippet: Metabolic profile of BPC-157 in two kinds of in vitro incubation models (HLM: human liver microsomes; HSS9: human skin S9).

Article Snippet: Mixed-gender human liver microsomes (10-Donor Pool, total protein concentration of 20 mg/mL) and mixed-gender human skin S9 (3-Donor Pool, total protein concentration of 1.89 mg/mL) were purchased from BioreclamationIVT (Westbury, NY, USA).

Techniques: In Vitro, Incubation

Journal: Molecules

Article Title: Stable Isotope Labeling-Based Nontargeted Strategy for Characterization of the In Vitro Metabolic Profile of a Novel Doping BPC-157 in Doping Control by UHPLC-HRMS

doi: 10.3390/molecules28217345

Figure Lengend Snippet: Identification of the suspected metabolite (BPC-157 (3–15) free acid, m / z 617.32733). ( a ) EIC of BPC-157 in human liver microsomes incubated sample; ( b ) EIC of m / z 617.32733 in human liver microsomes incubated sample; ( c ) EIC of m / z 617.32733 in enzyme blank sample; ( d ) EIC of the RM BPC-157 (3–15) free acid; ( e ) MS/MS spectrum of the RM BPC-157; ( f ) MS/MS spectrum of m / z 617.32733 in human liver microsomes incubated sample; ( g ) MS/MS spectrum of the RM BPC-157 (3–15) free acid.

Article Snippet: Mixed-gender human liver microsomes (10-Donor Pool, total protein concentration of 20 mg/mL) and mixed-gender human skin S9 (3-Donor Pool, total protein concentration of 1.89 mg/mL) were purchased from BioreclamationIVT (Westbury, NY, USA).

Techniques: Incubation, Tandem Mass Spectroscopy

Journal:

Article Title: Phage P22 procapsids equilibrate with free coat protein subunits

doi: 10.1016/j.jmb.2006.09.088

Figure Lengend Snippet: 35S-labeled coat protein monomers at 0.5 μM final were mixed with non-labeled coat protein shells at 0.006 and 0.012 μM shells. The samples were incubated at 20 °C and 41 °C for 1 day and separated by sucrose gradient sedimentation. The gradients were fractionated and the fractions were analyzed by liquid scintillation counting. The percent transfer was determined as described in the Materials and Methods. The error bars represent the standard deviation from 3 or 4 data sets

Article Snippet: The infected cultures were grown for 45 minutes, followed by additions of 1.875 μCi/mL of [ 35 S]-methionine and [ 35 S]-cysteine protein labeling mix (10 mCi/mL, NEN Life Science Products) every 30 minutes for 3 hours and 45 minutes.

Techniques: Labeling, Incubation, Sedimentation, Standard Deviation